Improved packing of poly(ethylenimine)/DNA complexes increases transfection efficiency
نویسندگان
چکیده
منابع مشابه
Improved transfection efficiency of 293 cells by radio frequency electroporation.
Electroporation, a widely used technique allowing the transfer of biological molecules into a variety of cell types (13), has been used with success for introducing plasmids and short oligonucleotides into mammalian, yeast and a variety of other cell types (1,2,9,10). A modification of the square-wave pulse technique that uses radio frequency (RF) electrical bursts is now being used effectively...
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BACKGROUND Physicochemical characteristics of liposome/DNA complexes influence transfection efficiency and affect each other in a very intricate way. The result of this is discrepancies in conclusions drawn about the individual influence of each one. METHODS Aiming to elucidate the influence of liposome/DNA charge ratio and size on transfection efficiency and on each other, we used liposome/D...
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A variety of methods have been described for introducing DNA into cultured mammalian cells. Among these, calcium phosphate (CaPO4)-mediated transfection has gained wide acceptance due to the procedure's low cost, simplicity and suitability for a wide range of cell types. However, this method is limited by relatively low transfection efficiencies and poor reproducibility. To improve the utility ...
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Efficient gene transfer into human pluripotent stem cells (hPSCs) holds great promise for regenerative medicine and pharmaceutical development. In the past decade, various methods were developed for gene transfer into hPSCs; however, hPSCs form tightly packed colonies, making gene transfer difficult. In this study, we established a stable culture method of hPSCs at a single-cell state to reduce...
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Mucopolysaccharidosis type I (MPS I) is an autosomal disease caused by alpha-l-iduronidase (IDUA) deficiency. This study used IDUA knockout mice as a model to evaluate whether parameters such as dose of plasmid and time of treatment could influence the transfection efficiency of complexes formed with PEGylated cationic nanoemulsions and plasmid (pIDUA), which contains the gene that encodes for ...
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ژورنال
عنوان ژورنال: Gene Therapy
سال: 1999
ISSN: 0969-7128,1476-5462
DOI: 10.1038/sj.gt.3300976